ABSTRACT
Objective:To investigate the effect of long non-coding BANCR on the migration and invasion of melanoma cells and its mechanism.Methods:qPCR was used to detect the expression of BANCR in melanoma patients and the relationship between clini-copathological data.Kaplan-Meier analysis the survival of melanoma patients with different expression of BANCR.Transwell invasion assay was used to detect the effect of BANCR on the invasive ability of melanoma cells.The effect of BANCR on the migration of melanoma cells was detected by scratch healing assay.Western blot was used to detect Wnt/β-catenin Signal pathway protein expression.Results:BANCR was highly expressed in melanoma,especially with the higher pathological stage or the lymph node metastasis.The higher expression of BANCR,the worse survival of melanoma patients.The inhibition of BANCR expression could reduce the invasion and migration ability of melanoma cells.The expression of β-catenin and c-myc protein in Wnt/β-catenin signaling pathway was down-regulated after silencing BANCR.Conclusion:Long non-coding BANCR was highly expressed in melanoma patients and was negatively correlated with survival time,it also regulates melanoma cell migration and invasion by activating the Wnt/β-catenin signaling pathway.
ABSTRACT
<p><b>BACKGROUND</b>Acne inversa (AI), also called hidradenitis suppurativa, is a chronic, inflammatory, recurrent skin disease of the hair follicle. Familial AI shows autosomal-dominant inheritance caused by mutations in the γ-secretase genes. This study was aimed to identify the specific mutations in the γ-secretase genes in two Chinese families with AI.</p><p><b>METHODS</b>In this study, two Chinese families with AI were investigated. All the affected individuals in the two families mainly manifested with multiple comedones, pitted scars, and a few inflammatory nodules on their face, neck, trunk, axilla, buttocks, upper arms, and thighs. Reticulate pigmentation in the flexures areas resembled Dowling-Degos disease clinically and pathologically. In addition, one of the affected individuals developed anal canal squamous cell carcinoma. Molecular mutation analysis of γ-secretase genes including PSENEN, PSEN1, and NCSTN was performed by polymerase chain reaction and direct DNA sequencing.</p><p><b>RESULTS</b>Two novel mutations of PSENEN gene were identified, including a heterozygous missense mutation c.194T>G (p.L65R) and a splice site mutation c.167-2A>G.</p><p><b>CONCLUSIONS</b>The identification of the two mutations could expand the spectrum of mutations in the γ-secretase genes underlying AI and provide valuable information for further study of genotype-phenotype correlations.</p>
Subject(s)
Female , Humans , Male , Middle Aged , Amyloid Precursor Protein Secretases , Genetics , DNA Mutational Analysis , Hidradenitis Suppurativa , Diagnosis , Genetics , Hyperpigmentation , Diagnosis , Membrane Proteins , Genetics , Mutation , Pedigree , Skin Abnormalities , Diagnosis , Skin Diseases, Genetic , Diagnosis , Skin Diseases, Papulosquamous , DiagnosisABSTRACT
This study was aimed to explore the apoptotic effect of Resveratrol (RES) on KG-1 cells and the expression of bcl-2/bax in vitro, and to clarify the possible mechanism of apoptotic effect of RES on leukemia cells. After treating with different concentrations of RES, the suppressive effect of RES on proliferation of KG-1 cells was analyzed by MTT method. Transmission electron microscope technique were used to detect the apoptosis status of KG-1 cells. The cell cycle and apoptosis percentage of KG-1 cells treated with RES were detected by flow cytometry. The expressions of bcl-2, bax mRNA and protein were assessed by semiquantitative RT-PCR and flow cytometry. The results showed that RES could obviously inhibit proliferation of KG-1 cells (p < 0.05). Compared with the control group, the cell number in S phase of KG-1 cells treated with RES increased (p < 0.05), the apoptosis rate enhanced significantly (p < 0.01) and the expression level was down-regulated, while expression level of bax was obviously up-regulated (p < 0.01). It is concluded that RES significantly induces apoptosis of KG-1 cells in vitro, which is probably related to the down-regulation of bcl-2 expression and up-regulation of bax expression.